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KMID : 0368419840270030139
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Journal of Plant Biology
1984 Volume.27 No. 3 p.139 ~ p.147
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Growth Responses of Petunia as Affected by Several Pollutants in Vitro
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白基燁/Paek, Kee Yoeup
崔聖烈/李宰旭/崔柱堅/Choi, Seong Lyeor/Lee, Jae Wook/Choi, Joo Kyeon
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Abstract
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This in vitro study was employed to clarify the effects of several pollutants, i.e. So_2, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and grwoth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. Na_2SO_3 levels of more than 200㎍/㎖ had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by Na_2SO_3 concentration of more than 400㎍/㎖ were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100 ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0 ppm was effective for fresh weiht increase in shoot tip culture, and 3.0 ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50㎍/㎖ compared to control. However the formation and growth of shoots were greatly inhibited with more than 400㎍/㎖ and roots were not produced at all.
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